Genotyping of the kappa-casein and beta-lactoglobulin genes in Anatolian water buffalo by PCR-RFLP
نویسندگان
چکیده
منابع مشابه
GENOTYPING OF PANDHARPURI BUFFALO FOR k-CASEIN USING PCR -RFLP
The objective of the study was to genotype Pandharpuri buffalo breeds for k-casein type by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). A 379-bp k-casein gene fragment was amplified using primers K1 (5’CAC GTC ACC CAC ACC CAC ATT TAT C-3’) and K2 (5’-TAA TTA GCC CAT TTC GCC TTC TCT GT-3’). DNA from 20 Pandharpuri buffaloes were extracted by phenol chloroform me...
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In this study, the genetic diversity of three milk protein genes namely beta-casein, kappacasein and beta-lactoglobulin was estimated in Turkish cattle breeds. Based on these genes, breeds in Turkey have been grouped as: 1) Eastern Anatolian Red, Anatolian Black and Southern Anatolian Red and 2) Turkish Grey, Turkish Holstein and Holstein Candidate Bulls. B alleles of the three studied genes, w...
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More than 80% of the total milk protein contains caseins that are to forms α-s1, α-s2, β-casein and kappa casein. Kappa casein is smaller than other milk proteins but due to have a role in size and stability of micelles. The present study describes polymorphism of kappa casein gene. This is the first study of kappa casein gene polymorphism in Najdi cattle of Iran. We used the polymerase chain r...
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The world is going through the debate on hazardous effects of consumption of A1 milk on human health by producing of hazardous chemical compound like beta casomorphin-7 and how this is not the case with A2. The aim of the present study was to genotype the existing population of crossbred Holstein-Friesian (HF) cattle for polymorphism in â-Casein gene (CSN2) using PCR-RFLP technique. Total of 47...
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Background: Milk proteins genes have been the focus of the researches as the candidate target genes that play a decisive role when animal breeding is desired.Objectives: In the present study, the transcriptional levels of Beta-lactoglobulin (BLG) and Alpha S1 casein (CSN1S1) genes were investigated during prenatal, milking and drying times in mammary glands of the Adani goats which showed...
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ژورنال
عنوان ژورنال: International Journal of Dairy Technology
سال: 2015
ISSN: 1364-727X
DOI: 10.1111/1471-0307.12257